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Label-free multidimensional information acquisition from optogenetically engineered cells using a graphene transistor
Li GX(李恭新); Yang J(杨佳); Yang WG(杨文广); Wang FF(王飞飞); Wang YC(王越超); Wang WX(王文学); Liu LQ(刘连庆)
作者部门机器人学研究室
发表期刊Nanoscale
ISSN2040-3364
2018
卷号10期号:5页码:2285-2290
收录类别SCI ; EI
EI收录号20180604766020
WOS记录号WOS:000424075400015
产权排序1
资助机构National Natural Science Foundation of China (Grant No. 61522312, 91748212 and 61673372)
摘要The optogenetic technique, which allows the manipulation of cellular activity patterns in space and time by light, has transformed the field of neuroscience. However, acquiring multidimensional optogenetic information remains challenging despite the fact that several cellular information detection methods have been proposed. Herein, we present a new method to acquire label-free multidimensional information from optogenetically engineered cells using a graphene transistor. Using a graphene film to form a strong densely packed layer with cells, the cellular action potentials were characterized as light-activated transistor conductance signals, which quantified the multidimensional optogenetic information. Based on this approach, some important cellular optogenetic information, including electrophysiological state, cell concentration, expression levels of opsin and response to variable light intensity, were also precisely detected. Furthermore, the graphene transistor was also used to distinguish cells expressing different channelrhodopsin-2 variants. Our study offers a general detection method of multidimensional optogenetic information for extending the applications of the optogenetic technique and provides a novel sensor for the development of future biological prosthetic devices. © 2018 The Royal Society of Chemistry.
语种英语
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文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/21527
专题机器人学研究室
通讯作者Wang WX(王文学); Liu LQ(刘连庆)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Key Laboratory of Advanced Process Control for Light Industry, Ministry of Education, Institute of Automation, Jiangnan University, Wuxi 214122, China
3.University of Chinese Academy of Science, Beijing 100049, China
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GB/T 7714
Li GX,Yang J,Yang WG,et al. Label-free multidimensional information acquisition from optogenetically engineered cells using a graphene transistor[J]. Nanoscale,2018,10(5):2285-2290.
APA Li GX.,Yang J.,Yang WG.,Wang FF.,Wang YC.,...&Liu LQ.(2018).Label-free multidimensional information acquisition from optogenetically engineered cells using a graphene transistor.Nanoscale,10(5),2285-2290.
MLA Li GX,et al."Label-free multidimensional information acquisition from optogenetically engineered cells using a graphene transistor".Nanoscale 10.5(2018):2285-2290.
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