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Label-free multidimensional information acquisition from optogenetically engineered cells using a graphene transistor
Li GX(李恭新); Yang J(杨佳); Yang WG(杨文广); Wang FF(王飞飞); Wang YC(王越超); Wang WX(王文学); Liu LQ(刘连庆)
Department机器人学研究室
Source PublicationNanoscale
ISSN2040-3364
2018
Volume10Issue:5Pages:2285-2290
Indexed BySCI ; EI
EI Accession number20180604766020
WOS IDWOS:000424075400015
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China
AbstractThe optogenetic technique, which allows the manipulation of cellular activity patterns in space and time by light, has transformed the field of neuroscience. However, acquiring multidimensional optogenetic information remains challenging despite the fact that several cellular information detection methods have been proposed. Herein, we present a new method to acquire label-free multidimensional information from optogenetically engineered cells using a graphene transistor. Using a graphene film to form a strong densely packed layer with cells, the cellular action potentials were characterized as light-activated transistor conductance signals, which quantified the multidimensional optogenetic information. Based on this approach, some important cellular optogenetic information, including electrophysiological state, cell concentration, expression levels of opsin and response to variable light intensity, were also precisely detected. Furthermore, the graphene transistor was also used to distinguish cells expressing different channelrhodopsin-2 variants. Our study offers a general detection method of multidimensional optogenetic information for extending the applications of the optogenetic technique and provides a novel sensor for the development of future biological prosthetic devices. © 2018 The Royal Society of Chemistry.
Language英语
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS KeywordNEURAL CIRCUITS ; CAENORHABDITIS-ELEGANS ; OPTICAL INTERROGATION ; PRESSURE SENSOR ; IN-VIVO ; CHANNELRHODOPSIN-2 ; PHOTODETECTOR ; NEUROSCIENCE ; NANOSCALE ; CHANNEL
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
Funding ProjectNational Natural Science Foundation of China[61522312] ; National Natural Science Foundation of China[91748212] ; National Natural Science Foundation of China[61673372]
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/21527
Collection机器人学研究室
Corresponding AuthorWang WX(王文学); Liu LQ(刘连庆)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Key Laboratory of Advanced Process Control for Light Industry, Ministry of Education, Institute of Automation, Jiangnan University, Wuxi 214122, China
3.University of Chinese Academy of Science, Beijing 100049, China
Recommended Citation
GB/T 7714
Li GX,Yang J,Yang WG,et al. Label-free multidimensional information acquisition from optogenetically engineered cells using a graphene transistor[J]. Nanoscale,2018,10(5):2285-2290.
APA Li GX.,Yang J.,Yang WG.,Wang FF.,Wang YC.,...&Liu LQ.(2018).Label-free multidimensional information acquisition from optogenetically engineered cells using a graphene transistor.Nanoscale,10(5),2285-2290.
MLA Li GX,et al."Label-free multidimensional information acquisition from optogenetically engineered cells using a graphene transistor".Nanoscale 10.5(2018):2285-2290.
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