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Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters
Li M(李密); Liu LQ(刘连庆); Xu, Xinning; Xing, Xiaojing; Dang D(党丹); Xi N(席宁); Wang YC(王越超)
作者部门机器人学研究室
关键词Atomic Force Microscopy Cell Mechanics Viscoelasticity Primary Cell Relaxation Time
发表期刊Journal of the Mechanical Behavior of Biomedical Materials
ISSN1751-6161
2018
卷号82页码:193-201
收录类别SCI ; EI
EI收录号20181404974444
WOS记录号WOS:000432508800023
产权排序1
资助机构National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; CAS FEA International Partnership Program for Creative Research Teams
摘要

Cell mechanics plays an important role in regulating the physiological activities of cells. The advent of atomic force microscopy (AFM) provides a novel powerful instrument for quantifying the mechanics of single cells at the nanoscale. The applications of AFM in single-cell mechanical assays in the past decade have significantly contributed to the field of cell and molecular biology. However, current AFM-based cellular mechanical studies are commonly carried out with fixed measurement parameters, which provides limited information about the dynamic cellular mechanical behaviors in response to the variable external stimuli. In this work, we utilized AFM to investigate cellular viscoelasticity (portrayed as relaxation time) with varying measurement parameters, including ramp rate and surface dwell time, on both cell lines and primary cells. The experimental results show that the obtained cellular relaxation times are remarkably dependent on the parameter surface dwell time and ramp rate during measurements. Besides, the dependencies to the measurement parameters are variable for different types of cells, which can be potentially used to indicate cell states. The research improves our understanding of single-cell dynamic rheology and provides a novel idea for discriminating different types of cells by AFM-based cellular viscoelastic assays with varying measurement parameters.

语种英语
WOS类目Engineering, Biomedical ; Materials Science, Biomaterials
关键词[WOS]BREAST-CANCER CELLS ; LIVING CELLS ; SINGLE-CELL ; MECHANICS ; BIOLOGY ; AFM ; MICROENVIRONMENT ; MICRORHEOLOGY ; GENERATION ; RHEOLOGY
WOS研究方向Engineering ; Materials Science
资助项目National Natural Science Foundation of China[61503372] ; National Natural Science Foundation of China[61522312] ; National Natural Science Foundation of China[U1613220] ; National Natural Science Foundation of China[61433017] ; National Natural Science Foundation of China[61504072] ; Youth Innovation Promotion Association CAS[2017243] ; CAS FEA International Partnership Program for Creative Research Teams
引用统计
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/21732
专题机器人学研究室
通讯作者Li M(李密); Liu LQ(刘连庆)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Department of Hematology, Liaoning Cancer Hospital, Shenyang 110042, China
3.School of Medical Devices, Shenyang Pharmaceutical University, Shenyang 110016, China
4.Department of Industrial and Manufacturing Systems Engineering, The University of Hong Kong, Hong Kong
推荐引用方式
GB/T 7714
Li M,Liu LQ,Xu, Xinning,et al. Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters[J]. Journal of the Mechanical Behavior of Biomedical Materials,2018,82:193-201.
APA Li M.,Liu LQ.,Xu, Xinning.,Xing, Xiaojing.,Dang D.,...&Wang YC.(2018).Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters.Journal of the Mechanical Behavior of Biomedical Materials,82,193-201.
MLA Li M,et al."Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters".Journal of the Mechanical Behavior of Biomedical Materials 82(2018):193-201.
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