SIA OpenIR  > 机器人学研究室
Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters
Li M(李密); Liu LQ(刘连庆); Xu, Xinning; Xing, Xiaojing; Dang D(党丹); Xi N(席宁); Wang YC(王越超)
Department机器人学研究室
Source PublicationJournal of the Mechanical Behavior of Biomedical Materials
ISSN1751-6161
2018
Volume82Pages:193-201
Indexed BySCI ; EI
EI Accession number20181404974444
WOS IDWOS:000432508800023
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; CAS FEA International Partnership Program for Creative Research Teams
KeywordAtomic Force Microscopy Cell Mechanics Viscoelasticity Primary Cell Relaxation Time
Abstract

Cell mechanics plays an important role in regulating the physiological activities of cells. The advent of atomic force microscopy (AFM) provides a novel powerful instrument for quantifying the mechanics of single cells at the nanoscale. The applications of AFM in single-cell mechanical assays in the past decade have significantly contributed to the field of cell and molecular biology. However, current AFM-based cellular mechanical studies are commonly carried out with fixed measurement parameters, which provides limited information about the dynamic cellular mechanical behaviors in response to the variable external stimuli. In this work, we utilized AFM to investigate cellular viscoelasticity (portrayed as relaxation time) with varying measurement parameters, including ramp rate and surface dwell time, on both cell lines and primary cells. The experimental results show that the obtained cellular relaxation times are remarkably dependent on the parameter surface dwell time and ramp rate during measurements. Besides, the dependencies to the measurement parameters are variable for different types of cells, which can be potentially used to indicate cell states. The research improves our understanding of single-cell dynamic rheology and provides a novel idea for discriminating different types of cells by AFM-based cellular viscoelastic assays with varying measurement parameters.

Language英语
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS KeywordBREAST-CANCER CELLS ; LIVING CELLS ; SINGLE-CELL ; MECHANICS ; BIOLOGY ; AFM ; MICROENVIRONMENT ; MICRORHEOLOGY ; GENERATION ; RHEOLOGY
WOS Research AreaEngineering ; Materials Science
Funding ProjectNational Natural Science Foundation of China[61503372] ; National Natural Science Foundation of China[61522312] ; National Natural Science Foundation of China[U1613220] ; National Natural Science Foundation of China[61433017] ; National Natural Science Foundation of China[61504072] ; Youth Innovation Promotion Association CAS[2017243] ; CAS FEA International Partnership Program for Creative Research Teams
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/21732
Collection机器人学研究室
Corresponding AuthorLi M(李密); Liu LQ(刘连庆)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Department of Hematology, Liaoning Cancer Hospital, Shenyang 110042, China
3.School of Medical Devices, Shenyang Pharmaceutical University, Shenyang 110016, China
4.Department of Industrial and Manufacturing Systems Engineering, The University of Hong Kong, Hong Kong
Recommended Citation
GB/T 7714
Li M,Liu LQ,Xu, Xinning,et al. Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters[J]. Journal of the Mechanical Behavior of Biomedical Materials,2018,82:193-201.
APA Li M.,Liu LQ.,Xu, Xinning.,Xing, Xiaojing.,Dang D.,...&Wang YC.(2018).Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters.Journal of the Mechanical Behavior of Biomedical Materials,82,193-201.
MLA Li M,et al."Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters".Journal of the Mechanical Behavior of Biomedical Materials 82(2018):193-201.
Files in This Item: Download All
File Name/Size DocType Version Access License
Nanoscale characteri(2819KB)期刊论文作者接受稿开放获取ODC PDDLView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li M(李密)]'s Articles
[Liu LQ(刘连庆)]'s Articles
[Xu, Xinning]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li M(李密)]'s Articles
[Liu LQ(刘连庆)]'s Articles
[Xu, Xinning]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li M(李密)]'s Articles
[Liu LQ(刘连庆)]'s Articles
[Xu, Xinning]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.