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题名:
Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters
作者: Li M(李密); Liu LQ(刘连庆); Xu, Xinning; Xing, Xiaojing; Dang D(党丹); Xi N(席宁); Wang YC(王越超)
作者部门: 机器人学研究室
通讯作者: Li M(李密) ; Liu LQ(刘连庆)
关键词: Atomic force microscopy ; Cell mechanics ; Viscoelasticity ; Primary cell ; Relaxation time
刊名: Journal of the Mechanical Behavior of Biomedical Materials
ISSN号: 1751-6161
出版日期: 2018
卷号: 82, 页码:193-201
收录类别: SCI ; EI
EI收录号: 20181404974444
WOS记录号: WOS:000432508800023
产权排序: 1
项目资助者: National Natural Science Foundation of China (61503372, 61522312, U1613220, 61433017, 61504072), the Youth Innovation Promotion Association CAS (No.2017243), and the CAS FEA International Partnership Program for Creative Research Teams
摘要: Cell mechanics plays an important role in regulating the physiological activities of cells. The advent of atomic force microscopy (AFM) provides a novel powerful instrument for quantifying the mechanics of single cells at the nanoscale. The applications of AFM in single-cell mechanical assays in the past decade have significantly contributed to the field of cell and molecular biology. However, current AFM-based cellular mechanical studies are commonly carried out with fixed measurement parameters, which provides limited information about the dynamic cellular mechanical behaviors in response to the variable external stimuli. In this work, we utilized AFM to investigate cellular viscoelasticity (portrayed as relaxation time) with varying measurement parameters, including ramp rate and surface dwell time, on both cell lines and primary cells. The experimental results show that the obtained cellular relaxation times are remarkably dependent on the parameter surface dwell time and ramp rate during measurements. Besides, the dependencies to the measurement parameters are variable for different types of cells, which can be potentially used to indicate cell states. The research improves our understanding of single-cell dynamic rheology and provides a novel idea for discriminating different types of cells by AFM-based cellular viscoelastic assays with varying measurement parameters.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/21732
Appears in Collections:机器人学研究室_期刊论文

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作者单位: 1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Department of Hematology, Liaoning Cancer Hospital, Shenyang 110042, China
3.School of Medical Devices, Shenyang Pharmaceutical University, Shenyang 110016, China
4.Department of Industrial and Manufacturing Systems Engineering, The University of Hong Kong, Hong Kong

Recommended Citation:
Li M,Liu LQ,Xu, Xinning,et al. Nanoscale characterization of dynamic cellular viscoelasticity by atomic force microscopy with varying measurement parameters[J]. Journal of the Mechanical Behavior of Biomedical Materials,2018,82:193-201.
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