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Regulation of C2C12 Differentiation and Control of the Beating Dynamics of Contractile Cells for a Muscle-Driven Biosyncretic Crawler by Electrical Stimulation
Liu LQ(刘连庆)1; Zhang C(张闯)1,2; Wang WX(王文学)1; Xi N(席宁)3; Wang YC(王越超)1
Department机器人学研究室
Source PublicationSOFT ROBOTICS
ISSN2169-5172
2018
Volume5Issue:6Pages:748-760
Indexed BySCI
WOS IDWOS:000447617900001
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China ; Key Research Program of Frontier Sciences, CAS ; CAS/SAFEA International Partnership Program for Creative Research Teams
KeywordSoft Robots Biosyncretic Robots Biohybrid Devices Living Machines C2c12 Differentiation
Abstract

Biosyncretic robots have potential advantages associated with both living organisms and electromechanical systems. Skeletal muscle tissue is a candidate as bioactuators for biosyncretic robots because of its excellent contraction force and controllability. However, the low quality of myoblast (C2C12) differentiation into contractile myotubes and the lack of control research on biosyncretic robots are two of the main challenges in the development of biosyncretic robots. In this study, an approach with circularly distributed multiple electrodes (CEs) is proposed to improve C2C12 differentiation and to control the movement of a myotube-based biosyncretic crawling robot. To analyze the advantages of the proposed CEs, the electrical characteristics of CEs and a pair of traditional parallel stimulation electrodes (PEs) were simulated and compared with each other. Then, to determine the optimal electrical stimulation parameters and demonstrate the superiorities of the proposed CEs, electrical pulses with different parameters were used to stimulate two-dimensional and three-dimensional cells during culture with the proposed CEs and PEs. After this the control characteristics of the muscle tissue by the CEs were investigated from the relevance of pulse width-threshold voltage, voltage-contractility, frequency-contractility, and electric field direction-contractility by measuring the real-time responses of myotubes to different electrical stimulations. Moreover, to demonstrate the control of biosyncretic robots by the CEs, a biomimetic biosyncretic crawler actuated by myotubes was designed, fabricated, and controlled to move at different speeds by varying directions of electric field. This study not only provides a potential tool for the development and control of biosyncretic robots but is also informative for muscle tissue engineering and cardiomyocyte culture.

Language英语
WOS SubjectRobotics
WOS KeywordSkeletal-muscle ; In-vitro ; Biological Machines ; Long-term ; Tissue ; Rat ; Myotubes ; Expression ; Actuators ; Force
WOS Research AreaRobotics
Funding ProjectNational Natural Science Foundation of China[61673372] ; National Natural Science Foundation of China[91748212] ; National Natural Science Foundation of China[61522312] ; National Natural Science Foundation of China[61433017] ; Key Research Program of Frontier Sciences, CAS[QYZDB-SSW-JSC008] ; CAS/SAFEA International Partnership Program for Creative Research Teams
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/23426
Collection机器人学研究室
Corresponding AuthorLiu LQ(刘连庆)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.University of Chinese Academy of Sciences, Beijing, China
3.Department of Industrial and Manufacturing Systems Engineering, Emerging Technologies Institute, University of Hong Kong Pokfulam, Hong Kong, Hong Kong
Recommended Citation
GB/T 7714
Liu LQ,Zhang C,Wang WX,et al. Regulation of C2C12 Differentiation and Control of the Beating Dynamics of Contractile Cells for a Muscle-Driven Biosyncretic Crawler by Electrical Stimulation[J]. SOFT ROBOTICS,2018,5(6):748-760.
APA Liu LQ,Zhang C,Wang WX,Xi N,&Wang YC.(2018).Regulation of C2C12 Differentiation and Control of the Beating Dynamics of Contractile Cells for a Muscle-Driven Biosyncretic Crawler by Electrical Stimulation.SOFT ROBOTICS,5(6),748-760.
MLA Liu LQ,et al."Regulation of C2C12 Differentiation and Control of the Beating Dynamics of Contractile Cells for a Muscle-Driven Biosyncretic Crawler by Electrical Stimulation".SOFT ROBOTICS 5.6(2018):748-760.
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