| Development of a novel optogenetic indicator based on cellular deformations for mapping optogenetic activities | |
Li GX(李恭新)1,2; Yang J(杨佳)2,3; Wang YC(王越超)2 ; Wang WX(王文学)2; iu LQ(刘连庆)2
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| Department | 机器人学研究室 |
| Source Publication | NANOSCALE
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| ISSN | 2040-3364 |
| 2018 | |
| Volume | 10Issue:45Pages:21046-21051 |
| Indexed By | SCI ; EI |
| EI Accession number | 20184906172492 |
| WOS ID | WOS:000451738900066 |
| Contribution Rank | 1 |
| Funding Organization | National Natural Science Foundation of China ; Research Program of Frontier Sciences, CAS ; Natural Science Foundation of Jiangsu Province of China |
| Abstract | Optogenetic techniques have changed the landscape of neuroscience by offering high temporal and spatial mapping of the activities of genetically defined population of cells with optical controlling tools. The mapping of optogenetic activities demands optogenetic indicators whose optical properties change in response to cellular activities, but the existing optogenetic indicators only specifically characterize limited optogenetic activities. Here, we propose a novel optogenetic indicator based on cellular deformation to characterize the activities of optogenetically engineered cells. The cellular activities triggered by light stimulation lead to changes in the cell membrane structure and result in cellular deformation, which is measured by atomic force microscopy. The deformation recordings of the cells expressing channelrhodopsin-2 (ChR2) and the corresponding control experiments together confirm that the deformation is generated generally when the cells are exposed to light, which is also validated indirectly via the change in the Young's modulus of the cells before and after absorption of photons. The activities of cells expressing different subtypes of opsins were also recorded using the optogenetic indicator of cellular deformation. This study provides a novel and general optogenetic indicator based on cellular deformation for monitoring the activities of optogenetically engineered cells. Moreover, this new optogenetic indicator offers ever-better tools for the applications of optogenetic activity mapping and neural and brain imaging. |
| Language | 英语 |
| WOS Subject | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied |
| WOS Keyword | NEURAL CIRCUITS ; CELLS ; CHANNELRHODOPSIN-2 |
| WOS Research Area | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| Funding Project | National Natural Science Foundation of China[61522312] ; National Natural Science Foundation of China[91748212] ; National Natural Science Foundation of China[61673372] ; Research Program of Frontier Sciences, CAS[QYZDB-SSW-JSC008] ; Natural Science Foundation of Jiangsu Province of China[BK20180592] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sia.cn/handle/173321/23672 |
| Collection | 机器人学研究室 |
| Corresponding Author | Wang WX(王文学) |
| Affiliation | 1.Key Laboratory of Advanced Process Control for Light Industry (Ministry of Education), Institute of Automation, Jiangnan University, Wuxi 214122, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shenyang 110016, China 3.University of the Chinese Academy of Sciences, Beijing 100049, China |
| Recommended Citation GB/T 7714 | Li GX,Yang J,Wang YC,et al. Development of a novel optogenetic indicator based on cellular deformations for mapping optogenetic activities[J]. NANOSCALE,2018,10(45):21046-21051. |
| APA | Li GX,Yang J,Wang YC,Wang WX,&iu LQ.(2018).Development of a novel optogenetic indicator based on cellular deformations for mapping optogenetic activities.NANOSCALE,10(45),21046-21051. |
| MLA | Li GX,et al."Development of a novel optogenetic indicator based on cellular deformations for mapping optogenetic activities".NANOSCALE 10.45(2018):21046-21051. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Development of a nov（1981KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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