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Development of a novel optogenetic indicator based on cellular deformations for mapping optogenetic activities
Li GX(李恭新)1,2; Yang J(杨佳)2,3; Wang YC(王越超)2; Wang WX(王文学)2; iu LQ(刘连庆)2
Department机器人学研究室
Source PublicationNANOSCALE
ISSN2040-3364
2018
Volume10Issue:45Pages:21046-21051
Indexed BySCI ; EI
EI Accession number20184906172492
WOS IDWOS:000451738900066
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China ; Research Program of Frontier Sciences, CAS ; Natural Science Foundation of Jiangsu Province of China
AbstractOptogenetic techniques have changed the landscape of neuroscience by offering high temporal and spatial mapping of the activities of genetically defined population of cells with optical controlling tools. The mapping of optogenetic activities demands optogenetic indicators whose optical properties change in response to cellular activities, but the existing optogenetic indicators only specifically characterize limited optogenetic activities. Here, we propose a novel optogenetic indicator based on cellular deformation to characterize the activities of optogenetically engineered cells. The cellular activities triggered by light stimulation lead to changes in the cell membrane structure and result in cellular deformation, which is measured by atomic force microscopy. The deformation recordings of the cells expressing channelrhodopsin-2 (ChR2) and the corresponding control experiments together confirm that the deformation is generated generally when the cells are exposed to light, which is also validated indirectly via the change in the Young's modulus of the cells before and after absorption of photons. The activities of cells expressing different subtypes of opsins were also recorded using the optogenetic indicator of cellular deformation. This study provides a novel and general optogenetic indicator based on cellular deformation for monitoring the activities of optogenetically engineered cells. Moreover, this new optogenetic indicator offers ever-better tools for the applications of optogenetic activity mapping and neural and brain imaging.
Language英语
WOS SubjectChemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied
WOS KeywordNEURAL CIRCUITS ; CELLS ; CHANNELRHODOPSIN-2
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
Funding ProjectNational Natural Science Foundation of China[61522312] ; National Natural Science Foundation of China[91748212] ; National Natural Science Foundation of China[61673372] ; Research Program of Frontier Sciences, CAS[QYZDB-SSW-JSC008] ; Natural Science Foundation of Jiangsu Province of China[BK20180592]
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Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/23672
Collection机器人学研究室
Corresponding AuthorWang WX(王文学)
Affiliation1.Key Laboratory of Advanced Process Control for Light Industry (Ministry of Education), Institute of Automation, Jiangnan University, Wuxi 214122, China
2.State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shenyang 110016, China
3.University of the Chinese Academy of Sciences, Beijing 100049, China
Recommended Citation
GB/T 7714
Li GX,Yang J,Wang YC,et al. Development of a novel optogenetic indicator based on cellular deformations for mapping optogenetic activities[J]. NANOSCALE,2018,10(45):21046-21051.
APA Li GX,Yang J,Wang YC,Wang WX,&iu LQ.(2018).Development of a novel optogenetic indicator based on cellular deformations for mapping optogenetic activities.NANOSCALE,10(45),21046-21051.
MLA Li GX,et al."Development of a novel optogenetic indicator based on cellular deformations for mapping optogenetic activities".NANOSCALE 10.45(2018):21046-21051.
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