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Quantifying The Adhesion Forces of Lymphoma Cells by AFM Single-cell Force Spectroscopy
其他题名基于AFM单细胞力谱技术的淋巴瘤细胞黏附力测量
Dang D(党丹)1,2; Xiang RW(项荣武)1; Liu B(刘斌)3,4; Liu XF (刘小菲)1; Li M(李密)3,4
作者部门机器人学研究室
关键词atomic force microscopy single-cell force spectroscopy cellular adhesion lymphoma cell rituximab
发表期刊生物化学与生物物理进展
ISSN1000-3282
2019
卷号46期号:1页码:89-98
收录类别SCI
WOS记录号WOS:000457065800010
产权排序3
资助机构National Natural Science Foundation of China (61873258, 61503372, 61433017) ; State Key Laboratory of Robotics(2016-O10)
摘要Cell adhesion plays an important role in regulating diverse physiological functions of cells,and quantitatively characterizing the adhesive behaviors at single-cell level benefits understanding the biology of cells.The advent of atomic force microscopy(AFM)provides a powerful method for investigating the biophysical properties of biological systems at micro/nanoscale under aqueous conditions,and particularly AFM-based single-cell force spectroscopy(SCFS)is able to measure the adhesion forces of single cells.Nevertheless,current SCFS assays are commonly performed on adherent cells,and SCFS studies on mammalian suspended cells are still scarce.In this work,AFM-based SCFS was utilized to measure the adhesion forces of lymphoma cells.First, the adhesion forces between lymphoma cells and rituximab(an antibody which binds to the CD20antigen on lymphoma cells to activate immunotherapy)were investigated.Then the effects of antibody concentration and experimental parameters on the adhesion force measurements were investigated.Next,the intercellular adhesion forces between lymphoma cells were quantified.The research demonstrates the capabilities of AFM-based SCFS in detecting the adhesive behaviors of mammalian suspended cells and also provides novel insights into the adhesion of lymphoma cells,which will have potential impacts on single-cell biomechanical assays.
语种英语
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文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/24133
专题机器人学研究室
通讯作者Dang D(党丹); Liu B(刘斌); Li M(李密)
作者单位1.School of Medical devices, Shenyang Pharmaceutical University, Shenyang 110016, China
2.College of Mechanical Engineering, Northeastern University, Shenyang 110819, China
3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
4.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China
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Dang D,Xiang RW,Liu B,et al. Quantifying The Adhesion Forces of Lymphoma Cells by AFM Single-cell Force Spectroscopy[J]. 生物化学与生物物理进展,2019,46(1):89-98.
APA Dang D,Xiang RW,Liu B,Liu XF ,&Li M.(2019).Quantifying The Adhesion Forces of Lymphoma Cells by AFM Single-cell Force Spectroscopy.生物化学与生物物理进展,46(1),89-98.
MLA Dang D,et al."Quantifying The Adhesion Forces of Lymphoma Cells by AFM Single-cell Force Spectroscopy".生物化学与生物物理进展 46.1(2019):89-98.
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