SIA OpenIR  > 机器人学研究室
Alternative TitleMeasurement and analysis of cellular viscoelastic properties using atomic force microscopy
党丹1; 李密2,3; 项荣武1
Source Publication科学通报
Indexed ByEI ; CSCD
EI Accession number20192607106221
Contribution Rank2
Funding Organization国家自然科学基金(61873258, 61503372) ; 中国科学院青年创新促进会项目(2017243)
Keyword原子力显微镜 细胞机械特性 压痕技术 黏弹特性 松弛时间
Other AbstractCell mechanics plays an important role in cellular physiological and pathological processes. During the formation and progression of tumors, the alterations in the mechanics of cancerous cells and tumor micro-environment promote the growth and migration of cancerous cells. Hence, investigating cell mechanics is of crucial significance in understanding the underlying mechanisms regulating life activities and diseases. The advent of atomic force microscopy (AFM) provides a powerful tool for detecting the mechanical properties of single cells. Compared with other single-cell mechanical analysis techniques, the advantage of AFM is that AFM is able to simultaneously obtain the topography and mechanics of cells, which is particularly useful for investigating the correlation between cell structures and cell mechanics. The biomedical applications of AFM in single-cell mechanics provide considerable novel insights into how cell and tissue mechanics affect tumor development and metastasis, contributing much to the communities of biomechanics and biophysics. However, current AFM single-cell mechanical experiments are commonly performed on measuring the elastic properties of cells and studies about the viscoelastic properties of cells are still scarce. In this work, AFM was utilized to measure and analyze the viscoelastic properties of cells. First, the detailed procedure of detecting the viscoelasticity of cells based on AFM indentation technique was established. AFM probe was controlled to perform approach-dwell-retract movement on cells in the vertical direction. During the approach-dwell-retract process, the deflection of AFM cantilever versus time was recorded, which yielded the force-time (F-T) curves. The original F-T curves were then normalized and fitted by two-order Maxwell model, which gave two cellular relaxation times (the first relaxation time τ1 and the second relaxation time τ2). The fitting results showed that the theoretical curve matched the experimental relaxation curve well, indicating that the two-order Maxwell model was suited for characterizing the relaxation behaviors of cells. Second, the established procedure was used to measure the relaxation time of six different types of cells, including mammalian adherent cells, mammalian suspended cells, normal cells, cancerous cells, cell lines cultured in vitro and primary cells prepared from bone marrow and peripheral blood of healthy volunteers. The fitting curves were consistent with the experimental relaxation curves for all of the six types of cells used here, indicating the effectiveness of the presented method for detecting the viscoelastic properties of cells. Besides, the results showed the various cellular relaxation times for the different types of cells. The statistical results showed that the first cellular relaxation times were in the range 0.01−0.03 s, which might correspond to the behaviors of cytoplasm. The second cellular relaxation time was in the range 0.2−0.4 s, which might correspond to the behaviors of the cytoskeleton. Finally, regression analysis was performed on the measured cellular relaxation times, showing the linear relationship between the first cellular relaxation time τ1 and the second cellular relaxation time τ2, and the regression coefficients were variable for different types of cells. The research improves our understanding of cellular viscoelasticity and also provides a novel idea to measure the viscoelastic properties of cells, which will have potential impacts on cell mechanics and biomedicine.
Citation statistics
Document Type期刊论文
Corresponding Author党丹; 李密
Recommended Citation
GB/T 7714
党丹,李密,项荣武. 基于原子力显微镜(AFM)的细胞黏弹特性测量与分析[J]. 科学通报,2019,64(15):1610-1619.
APA 党丹,李密,&项荣武.(2019).基于原子力显微镜(AFM)的细胞黏弹特性测量与分析.科学通报,64(15),1610-1619.
MLA 党丹,et al."基于原子力显微镜(AFM)的细胞黏弹特性测量与分析".科学通报 64.15(2019):1610-1619.
Files in This Item:
File Name/Size DocType Version Access License
基于原子力显微镜_AFM_的细胞黏弹特性(10949KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[党丹]'s Articles
[李密]'s Articles
[项荣武]'s Articles
Baidu academic
Similar articles in Baidu academic
[党丹]'s Articles
[李密]'s Articles
[项荣武]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[党丹]'s Articles
[李密]'s Articles
[项荣武]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 基于原子力显微镜_AFM_的细胞黏弹特性测量与分析.pdf
Format: Adobe PDF
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.