| Light-sheet microscopy in the near-infrared II window | |
Wang FF(王飞飞)1; Wan, Hao1; Ma, Zhuoran1; Zhong, Yeteng1; Sun, Qinchao1; Tian, Ye1; Qu, Liangqiong2; Du, Haotian1; Zhang, Mingxi3; Li, Lulin4; Ma, Huilong5; Luo, Jian4,6; Liang, Yongye5; Li WJ(李文荣)7,8; Hong, Guosong9; Liu LQ(刘连庆)7 ; Dai, Hongjie1
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| Department | 机器人学研究室 |
| Source Publication | NATURE METHODS
 |
| ISSN | 1548-7091 |
| 2019 | |
| Volume | 16Issue:6Pages:545-552 |
| Indexed By | SCI |
| WOS ID | WOS:000469455200024 |
| Contribution Rank | 7 |
| Funding Organization | National Institutes of Health |
| Abstract | Non-invasive deep-tissue three-dimensional optical imaging of live mammals with high spatiotemporal resolution is challenging owing to light scattering. We developed near-infrared II (1,000-1,700 nm) light-sheet microscopy with excitation and emission of up to approximately 1,320 nm and 1,700 nm, respectively, for optical sectioning at a penetration depth of approximately 750 mu m through live tissues without invasive surgery and at a depth of approximately 2 mm in glycerol-cleared brain tissues. Near-infrared II light-sheet microscopy in normal and oblique configurations enabled in vivo imaging of live mice through intact tissue, revealing abnormal blood flow and T-cell motion in tumor microcirculation and mapping out programmed-death ligand 1 and programmed cell death protein 1 in tumors with cellular resolution. Three-dimensional imaging through the intact mouse head resolved vascular channels between the skull and brain cortex, and allowed monitoring of recruitment of macrophages and microglia to the traumatic brain injury site. |
| Language | 英语 |
| WOS Subject | Biochemical Research Methods |
| WOS Keyword | IN-VIVO ; FLUORESCENCE MICROSCOPY ; 2-PHOTON MICROSCOPY ; DEEP ; BRAIN ; TISSUE ; LASER ; DYE ; NANOPARTICLES ; WAVELENGTHS |
| WOS Research Area | Biochemistry & Molecular Biology |
| Funding Project | National Institutes of Health[DP1-NS-105737] ; National Institutes of Health[DP1-NS-105737] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sia.cn/handle/173321/24725 |
| Collection | 机器人学研究室 |
| Corresponding Author | Dai, Hongjie |
| Affiliation | 1.Department of Chemistry and Bio-X, Stanford University, Stanford, CA, USA 2.Department of Radiology and BRIC, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA 3.State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, China 4.Palo Alto Veterans Institute for Research, VA Palo Alto Health Care System, Palo Alto, CA, USA 5.Department of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen, China 6.Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA 7.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China 8.Department of Mechanical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong 9.Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA. |
| Recommended Citation GB/T 7714 | Wang FF,Wan, Hao,Ma, Zhuoran,et al. Light-sheet microscopy in the near-infrared II window[J]. NATURE METHODS,2019,16(6):545-552. |
| APA | Wang FF.,Wan, Hao.,Ma, Zhuoran.,Zhong, Yeteng.,Sun, Qinchao.,...&Dai, Hongjie.(2019).Light-sheet microscopy in the near-infrared II window.NATURE METHODS,16(6),545-552. |
| MLA | Wang FF,et al."Light-sheet microscopy in the near-infrared II window".NATURE METHODS 16.6(2019):545-552. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Light-sheet microsco（3477KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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