SIA OpenIR  > 机器人学研究室
Light-sheet microscopy in the near-infrared II window
Wang FF(王飞飞)1; Wan, Hao1; Ma, Zhuoran1; Zhong, Yeteng1; Sun, Qinchao1; Tian, Ye1; Qu, Liangqiong2; Du, Haotian1; Zhang, Mingxi3; Li, Lulin4; Ma, Huilong5; Luo, Jian4,6; Liang, Yongye5; Li WJ(李文荣)7,8; Hong, Guosong9; Liu LQ(刘连庆)7; Dai, Hongjie1
Source PublicationNATURE METHODS
Indexed BySCI
WOS IDWOS:000469455200024
Contribution Rank7
Funding OrganizationNational Institutes of Health
AbstractNon-invasive deep-tissue three-dimensional optical imaging of live mammals with high spatiotemporal resolution is challenging owing to light scattering. We developed near-infrared II (1,000-1,700 nm) light-sheet microscopy with excitation and emission of up to approximately 1,320 nm and 1,700 nm, respectively, for optical sectioning at a penetration depth of approximately 750 mu m through live tissues without invasive surgery and at a depth of approximately 2 mm in glycerol-cleared brain tissues. Near-infrared II light-sheet microscopy in normal and oblique configurations enabled in vivo imaging of live mice through intact tissue, revealing abnormal blood flow and T-cell motion in tumor microcirculation and mapping out programmed-death ligand 1 and programmed cell death protein 1 in tumors with cellular resolution. Three-dimensional imaging through the intact mouse head resolved vascular channels between the skull and brain cortex, and allowed monitoring of recruitment of macrophages and microglia to the traumatic brain injury site.
WOS SubjectBiochemical Research Methods
WOS Research AreaBiochemistry & Molecular Biology
Funding ProjectNational Institutes of Health[DP1-NS-105737] ; National Institutes of Health[DP1-NS-105737]
Citation statistics
Cited Times:37[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorDai, Hongjie
Affiliation1.Department of Chemistry and Bio-X, Stanford University, Stanford, CA, USA
2.Department of Radiology and BRIC, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
3.State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, China
4.Palo Alto Veterans Institute for Research, VA Palo Alto Health Care System, Palo Alto, CA, USA
5.Department of Materials Science and Engineering, South University of Science and Technology of China, Shenzhen, China
6.Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA
7.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
8.Department of Mechanical Engineering, City University of Hong Kong, Kowloon Tong, Hong Kong
9.Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Recommended Citation
GB/T 7714
Wang FF,Wan, Hao,Ma, Zhuoran,et al. Light-sheet microscopy in the near-infrared II window[J]. NATURE METHODS,2019,16(6):545-552.
APA Wang FF.,Wan, Hao.,Ma, Zhuoran.,Zhong, Yeteng.,Sun, Qinchao.,...&Dai, Hongjie.(2019).Light-sheet microscopy in the near-infrared II window.NATURE METHODS,16(6),545-552.
MLA Wang FF,et al."Light-sheet microscopy in the near-infrared II window".NATURE METHODS 16.6(2019):545-552.
Files in This Item:
File Name/Size DocType Version Access License
Light-sheet microsco(3477KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang FF(王飞飞)]'s Articles
[Wan, Hao]'s Articles
[Ma, Zhuoran]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang FF(王飞飞)]'s Articles
[Wan, Hao]'s Articles
[Ma, Zhuoran]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang FF(王飞飞)]'s Articles
[Wan, Hao]'s Articles
[Ma, Zhuoran]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Light-sheet microscopy in the near-infrared II window.pdf
Format: Adobe PDF
All comments (0)
No comment.

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.