SIA OpenIR  > 机器人学研究室
Measurement of Radial Elasticity and Original Height of DNA Duplex Using Tapping-Mode Atomic Force Microscopy
Li LH(李龙海); Zhang, Xu; Wang, Hongfei; Lang, Qian; Chen HT(陈海涛); Liu LQ(刘连庆)
Department机器人学研究室
Source PublicationNANOMATERIALS
ISSN2079-4991
2019
Volume9Issue:4Pages:1-10
Indexed BySCI
WOS IDWOS:000467768800077
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China ; University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province ; China Postdoctoral Science Foundation
KeywordDNA duplex Young's modulus AFM tapping mode
AbstractAtomic force microscopy (AFM) can characterize nanomaterial elasticity. However, some one-dimensional nanomaterials, such as DNA, are too small to locate with an AFM tip because of thermal drift and the nonlinearity of piezoelectric actuators. In this study, we propose a novel approach to address the shortcomings of AFM and obtain the radial Young's modulus of a DNA duplex. The elastic properties are evaluated by combining physical calculations and measured experimental results. The initial elasticity of the DNA is first assumed; based on tapping-mode scanning images and tip-sample interaction force simulations, the calculated elastic modulus is extracted. By minimizing the error between the assumed and experimental values, the extracted elasticity is assigned as the actual modulus for the material. Furthermore, tapping-mode image scanning avoids the necessity of locating the probe exactly on the target sample. In addition to elasticity measurements, the deformation caused by the tapping force from the AFM tip is compensated and the original height of the DNA is calculated. The results show that the radial compressive Young's modulus of DNA is 125-150 MPa under a tapping force of 0.5-1.3 nN; its original height is 1.9 nm. This approach can be applied to the measurement of other nanomaterials.
Language英语
WOS SubjectNanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS KeywordPROBE
WOS Research AreaScience & Technology - Other Topics ; Materials Science
Funding ProjectNational Natural Science Foundation of China[31701311] ; University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province[UNPYSCT-2017016] ; China Postdoctoral Science Foundation[2017M621234] ; National Natural Science Foundation of China[31701311] ; University Nursing Program for Young Scholars with Creative Talents in Heilongjiang Province[UNPYSCT-2017016] ; China Postdoctoral Science Foundation[2017M621234]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/24731
Collection机器人学研究室
Corresponding AuthorChen HT(陈海涛); Liu LQ(刘连庆)
Affiliation1.College of Engineering, Northeast Agricultural University, Harbin 150030, China
2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
Recommended Citation
GB/T 7714
Li LH,Zhang, Xu,Wang, Hongfei,et al. Measurement of Radial Elasticity and Original Height of DNA Duplex Using Tapping-Mode Atomic Force Microscopy[J]. NANOMATERIALS,2019,9(4):1-10.
APA Li LH,Zhang, Xu,Wang, Hongfei,Lang, Qian,Chen HT,&Liu LQ.(2019).Measurement of Radial Elasticity and Original Height of DNA Duplex Using Tapping-Mode Atomic Force Microscopy.NANOMATERIALS,9(4),1-10.
MLA Li LH,et al."Measurement of Radial Elasticity and Original Height of DNA Duplex Using Tapping-Mode Atomic Force Microscopy".NANOMATERIALS 9.4(2019):1-10.
Files in This Item: Download All
File Name/Size DocType Version Access License
Measurement of Radia(2224KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Li LH(李龙海)]'s Articles
[Zhang, Xu]'s Articles
[Wang, Hongfei]'s Articles
Baidu academic
Similar articles in Baidu academic
[Li LH(李龙海)]'s Articles
[Zhang, Xu]'s Articles
[Wang, Hongfei]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Li LH(李龙海)]'s Articles
[Zhang, Xu]'s Articles
[Wang, Hongfei]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Measurement of Radial Elasticity and Original Height of DNA Duplex Using Tapping-Mode Atomic Force Microscopy.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.