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Mask-free generation of multicellular 3D heterospheroids array for high-throughput combinatorial anti-cancer drug screening
Yang WG(杨文广)1; Cai SX(蔡树向)1; Yuan, Z.1; Lai YB(来佑彬)2; Yu HB(于海波)3,4; Wang YC(王越超)3,4; Liu LQ(刘连庆)3,4
Department机器人学研究室
Source PublicationMaterials and Design
ISSN0264-1275
2019
Volume183Pages:1-8
Indexed BySCI ; EI
EI Accession number20193707417874
WOS IDWOS:000490732800032
Contribution Rank3
Funding OrganizationNational Natural Science Foundation of China (Project No. 61803323 and 51605311) ; Shandong Province Natural Science Foundation (Project No. ZR2019BF049)
Keyword3D heterospheroids generation microwells fabrication drug screening
Abstract

Since cancer incidence and mortality are rapidly growing worldwide, it is imperative to develop the effective tumor models in vitro for anti-cancer drug screening. Cellular spheroid as an excellent simple 3D model can bridge the gap between cell culture and live tissue. Although numerous approaches for generating cellular spheroids emerges, the long cultivation time and the heterospheroids formation with multiple cell types are still challenging. In the present study, a mask-free method of multicellular heterospheroids arrays formation using digital micromirror device (DMD)-based hydrogel fabricating system and microfluidic system is proposed. Microwell arrays were designed and manufactured online based on shadowed light which shows high flexible. Besides, heterospheroids with multiple cells controlled in different ratios can be generated and cultured for a long time in microfluidic system. Furthermore, the drug screening shows that heterospheroids have high rate of drug resistance compared with homospheroids and combinatorial drugs works better than the single drugs. These studies provide a 3D heterospheroids generating process for high-throughput combinatorial anti-cancer drug screening with potential cancer therapeutic application.

Language英语
WOS SubjectMaterials Science, Multidisciplinary
WOS KeywordSPHEROIDS ; FABRICATION ; CULTURE ; CHIP
WOS Research AreaMaterials Science
Funding ProjectNational Natural Science Foundation of China[61803323] ; National Natural Science Foundation of China[51605311] ; Shandong Province Natural Science Foundation[ZR2019BF049]
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/25626
Collection机器人学研究室
Corresponding AuthorYang WG(杨文广); Liu LQ(刘连庆)
Affiliation1.School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China
2.College of Engineering, Shenyang Agricultural University, Shenyang 110016, China
3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
4.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China
Recommended Citation
GB/T 7714
Yang WG,Cai SX,Yuan, Z.,et al. Mask-free generation of multicellular 3D heterospheroids array for high-throughput combinatorial anti-cancer drug screening[J]. Materials and Design,2019,183:1-8.
APA Yang WG.,Cai SX.,Yuan, Z..,Lai YB.,Yu HB.,...&Liu LQ.(2019).Mask-free generation of multicellular 3D heterospheroids array for high-throughput combinatorial anti-cancer drug screening.Materials and Design,183,1-8.
MLA Yang WG,et al."Mask-free generation of multicellular 3D heterospheroids array for high-throughput combinatorial anti-cancer drug screening".Materials and Design 183(2019):1-8.
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