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Nanotopographical Surfaces for Regulating Cellular Mechanical Behaviors Investigated by Atomic Force Microscopy
Li M(李密)1,2; Xi N(席宁)3; Wang YC(王越超)1,2; Liu LQ(刘连庆)1,2
Department机器人学研究室
Source PublicationACS BIOMATERIALS SCIENCE & ENGINEERING
ISSN2373-9878
2019
Volume5Issue:10Pages:5036-5050
Indexed BySCI ; EI
EI Accession number20194507646122
WOS IDWOS:000490658800015
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China [61922081, 61873258, U1613220] ; Youth Innovation Promotion Association Chinese Academy of Sciences [2017243]
Keywordatomic force microscopy cell-substrate interactions nanotopographical surface nanogranular deposition surface roughness mechanical properties
Abstract

Cell substrate interactions play an important role in regulating cellular physiological and pathological processes, and therefore, investigating cell substrate interface is meaningful for understanding the behaviors of cells. However, so far, the underlying mechanisms which guide the nanoscopic biological activities taking place at the cell-substrate interface remain poorly understood. The advent of atomic force microscopy (AFM) provides a powerful tool for characterizing the structures and properties of native biological and biomaterial systems with unprecedented spatiotemporal resolution, which offers new possibilities for understanding the physical sciences of biomaterials. Here, AFM was utilized to unravel the nanotopographical surfaces for regulating cellular behaviors on three different substrates (glass slide, mica, and Petri dish). First, the decellularized substrates prepared with the use of ammonia and trypsin were imaged by AFM, significantly showing the nanogranular substances on the decellularized substrates as well as the cell membrane patches for uncovering the detailed situations of mechanical contact between cells and substrates. Next, experiments performed on chemically fixed substrates with the use of paraformaldehyde together with AFM time-lapse imaging remarkably showed that nanogranular depositions from the cell culture medium appeared on the substrates for promoting cell growth. Further, the detailed cell culture medium components which contribute to the nanogranular depositions are identified. Finally, the dynamic alterations in surface roughness and mechanical properties of substrates and cells during cell growth were quantitatively measured by AFM, revealing the diverse changes of the multiple physical properties (surface roughness, adhesion force, Young's modulus, and relaxation time) during cell-substrate interactions. The research provides novel insights into the nanotopographical surfaces for cell-substrate interactions, which will be useful for understanding cellular behaviors.

Language英语
WOS SubjectMaterials Science, Biomaterials
WOS KeywordEXTRACELLULAR-MATRIX ; STEM-CELLS ; PHYSICAL INTERACTIONS ; PRACTICAL GUIDE ; CANCER ; NANOSCALE ; ADHESION ; MICROENVIRONMENT ; TOPOGRAPHY ; SUBSTRATUM
WOS Research AreaMaterials Science
Funding ProjectNational Natural Science Foundation of China[61922081] ; National Natural Science Foundation of China[61873258] ; National Natural Science Foundation of China[U1613220] ; Youth Innovation Promotion Association Chinese Academy of Sciences[2017243]
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/25776
Collection机器人学研究室
Corresponding AuthorLi M(李密); Liu LQ(刘连庆)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation
2.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China
3.Department of Industrial and Manufacturing Systems Engineering, The University of Hong Kong, Kowloon 999077, Hong Kong, China
Recommended Citation
GB/T 7714
Li M,Xi N,Wang YC,et al. Nanotopographical Surfaces for Regulating Cellular Mechanical Behaviors Investigated by Atomic Force Microscopy[J]. ACS BIOMATERIALS SCIENCE & ENGINEERING,2019,5(10):5036-5050.
APA Li M,Xi N,Wang YC,&Liu LQ.(2019).Nanotopographical Surfaces for Regulating Cellular Mechanical Behaviors Investigated by Atomic Force Microscopy.ACS BIOMATERIALS SCIENCE & ENGINEERING,5(10),5036-5050.
MLA Li M,et al."Nanotopographical Surfaces for Regulating Cellular Mechanical Behaviors Investigated by Atomic Force Microscopy".ACS BIOMATERIALS SCIENCE & ENGINEERING 5.10(2019):5036-5050.
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