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Development of Multi-Dimensional Cell Co-Culture via a Novel Microfluidic Chip Fabricated by DMD-Based Optical Projection Lithography
Ge ZX(葛治星)1,2,3; Yu HB(于海波)1,2; Yang WG(杨文广)4; Yang J(杨佳)1,2,3; Liu B(刘斌)1,2; Wang XD(王晓朵)1,2; Liu Z(刘柱)1,2; Liu LQ(刘连庆)1,2
Department机器人学研究室
Source PublicationIEEE TRANSACTIONS ON NANOBIOSCIENCE
ISSN1536-1241
2019
Volume18Issue:4Pages:679-686
Indexed BySCI ; EI
EI Accession number20194407607048
WOS IDWOS:000498049700019
Contribution Rank1
Funding OrganizationNational Key R&D Program of China [2018YFB1304900] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [61727811, 61803366, 91748212, 61803323, U1613220] ; CAS FEA International Partnership Program for Creative Research Teams ; Youth Innovation Promotion Association CAS [2015160]
KeywordMicrofluidic chip cellular behavior surface tension multi-dimensional cell co-culture
Abstract

Establishing a physiological microenvironment in vitro that is suitable for cell and tissue growth is essential for medical research. Microfluidic chips are widely used in the construction of a microenvironment and the analysis of cell behavior in vitro; however, the design and manufacture of microfluidic chips for the long-term culture of a tumor model tends to be highly complex and time-consuming. In this paper, we propose a method for the rapid fabrication of a microfluidic chip for multi-dimensional cell co-culture. A major advantage of this method is that the microfluidic chip can be divided into several sections by micro-pillar arrays to form different functional regions to grow two- and three-dimensional cell culture on the same matrix. At the micro-scale, the surface tension between the gelatin methacryloyl-encapsulated cells and micro-pillars prevents the leakage of the hydrogel, and the hydrogel provides a three-dimensional microenvironment for cell growth. Our results of long-term cell culture and preclinical drug screening showed that cells cultured in a two-dimensional monolayer differ from three-dimensional cultured cells in terms of morphology, area, survival rate, proliferation, and drug resistance. This method shows potential for use in the study of cell behavior, drug screening, and tissue engineering.

Language英语
WOS SubjectBiochemical Research Methods ; Nanoscience & Nanotechnology
WOS KeywordBREAST-CANCER ; INVASION ; MICROSTRUCTURES ; BEHAVIORS ; SYSTEM
WOS Research AreaBiochemistry & Molecular Biology ; Science & Technology - Other Topics
Funding ProjectNational Key R&D Program of China[2018YFB1304900] ; National Natural Science Foundation of China[61727811] ; National Natural Science Foundation of China[61803366] ; National Natural Science Foundation of China[91748212] ; National Natural Science Foundation of China[61803323] ; National Natural Science Foundation of China[U1613220] ; CAS FEA International Partnership Program for Creative Research Teams ; Youth Innovation Promotion Association CAS[2015160]
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/25924
Collection机器人学研究室
Corresponding AuthorYu HB(于海波); Liu LQ(刘连庆)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences (CAS), Shenyang 110016, China
2.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China
3.Institutes for Robotics and Intelligent Manufacturing, University of Chinese Academy of Sciences, Beijing 100049, China
4.School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China
Recommended Citation
GB/T 7714
Ge ZX,Yu HB,Yang WG,et al. Development of Multi-Dimensional Cell Co-Culture via a Novel Microfluidic Chip Fabricated by DMD-Based Optical Projection Lithography[J]. IEEE TRANSACTIONS ON NANOBIOSCIENCE,2019,18(4):679-686.
APA Ge ZX.,Yu HB.,Yang WG.,Yang J.,Liu B.,...&Liu LQ.(2019).Development of Multi-Dimensional Cell Co-Culture via a Novel Microfluidic Chip Fabricated by DMD-Based Optical Projection Lithography.IEEE TRANSACTIONS ON NANOBIOSCIENCE,18(4),679-686.
MLA Ge ZX,et al."Development of Multi-Dimensional Cell Co-Culture via a Novel Microfluidic Chip Fabricated by DMD-Based Optical Projection Lithography".IEEE TRANSACTIONS ON NANOBIOSCIENCE 18.4(2019):679-686.
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