SIA OpenIR  > 机器人学研究室
Alternative TitleDielectric Microsphere-based Real-time and Dynamic Super-resolution Microscopy System and Applications
Thesis Advisor李文荣 ; 刘连庆
Keyword介电微球透镜 超分辨成像 动态实时 微透镜阵列 倏逝波移频效应
Degree Discipline机械电子工程
Degree Name博士
Degree Grantor中国科学院沈阳自动化研究所
Place of Conferral沈阳
Other AbstractConsidering the problems exist in the physical mechanism and the large-scale, real-time super-resolution imaging applications of dielectric microsphere superlens, the research work of this dissertation combined the multidisciplinary techniques including micro/nano-optics, microfluidic technologies and engineering methods, and carried out the following studies based on theoretical analyses and experimental verifications: (1) Super-resolution imaging mechanism of dielectric microsphere: The fundamental mechanisms of microsphere-based super-resolution imaging are hot debates since its first demonstration. But the theoretical resolutions estimated from these existing mechanisms did not match very well with the experimental resolutions obtained. Based on systematical studies of the imaging properties of dielectric microsphere lens, we analyzed the effect of near-field evanescent wave of microsphere on its superresolution imaging. We proposed a combined role of evanescent wave illumination frequency-shift effect and coupling and transferring sample evanescent wave in the sub-diffraction-limit resolution. Our analyzed resolution based on annular spectrum study is in consistent with the experimental resolution. Based on the established imaging mechanism, we propose to use high-refractive-index dielectric nanoparticles-assembled micro-hemispheric lens to further improve the resolution. Based on theoretical simulation analysis, dielectric nanoparticles cascade transfer the confined light field onto sample surface, thus increasing the evanescent wave illumination spots and further enhancing the imaging resolution and contrast of the microlens. (2) Dielectric microsphere-array (MSA) flexible module: To further improve the imaging area and efficiency of microsphere-based super-resolution imaging, we proposed an air/solution interface induced self-assembly method to fabricate large-scale, high-quality uniformly distributed MSA. By immersing the MSA in flexible elastomers, we developed and demonstrated standard super-resolution optical modules with great reproducibility, which could be compatible with other systems. (3) Synchronous super-resolution observation of micro/nano manufacturing based on MSA: aiming at the limitations of other nano-imaging technologies, such as off-line and fluorescence-related, we carried out in-situ and real-time super-resolution observation of the dynamic deposition processes of metallic nanoparticles and thin-films micro/nano manufacturing system based on dielectric MSA lenses, and realized synchronous nano-observation of micro/nano manufacturing under white light condition. (4) Non-invasive, real-time super-resolution imaging of biological subcellular dynamics: Aiming at the problems of fluorescence super-resolution imaging technologies, such as high radiation intensity and fluorescence-induced phototoxicity, we conducted long-term, non-invasive, real-time observations of live-cell dynamics by simply upgrading the conventional microscope system by the MSA superlens thin-film. The migration activities of subcellular vesicles were observed, which laid a foundation for further study on the dynamic generation and reconstruction of subcellular structures and biological processes, and the essence of life at micro/nano scale. In this dissertation, the research work provides theoretical support for dielectric microsphere based super-resolution imaging technology, and also provides relevant technical support for realizing large-scale, high-efficiency real-time and dynamic nano-observation, which will promote the ultra-precision micro/nano-manufacturing, personalized medicine and other related fields.
Contribution Rank1
Document Type学位论文
Recommended Citation
GB/T 7714
李盼. 基于介电微球透镜的实时动态光学超分辨成像系统及应用研究[D]. 沈阳. 中国科学院沈阳自动化研究所,2019.
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