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Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins
Li M(李密)1,2,3; Wang YC(王越超)1,2,3; Liu LQ(刘连庆)1,2,3
Department机器人学研究室
Source PublicationIEEE TRANSACTIONS ON NANOBIOSCIENCE
ISSN1536-1241
2020
Volume19Issue:1Pages:78-99
Indexed BySCI ; EI ; CPCI(ISTP)
EI Accession number20200308036270
WOS IDWOS:000506601200009
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China [61922081, 61873258, U1613220] ; Research Program of Frontier Sciences CAS [ZDBS-LY-JSC043] ; Youth Innovation Promotion Association CAS [2017243]
KeywordAtomic force microscopy (AFM) single-protein analysis morphological imaging single-molecule force recognition mechanical unfolding high-speed AFM
Abstract

Proteins play a crucial role in regulating life activities and therefore investigating the behaviors of proteins is of critical significance for understanding the underlying mechanisms guiding the physiological and pathological processes of living organisms. Traditional molecular biochemical methods for protein assays are based on ensemble measurements which reflect the averaged behaviors of the whole molecular populations, veiling the rare activities of small molecular subpopulations. Achievements obtained in the past decades have proved that atomic force microscopy (AFM) is a powerful multifunctional tool for characterizing the behaviors of single proteins at work in aqueous conditions with unprecedented spatiotemporal resolution, which provides novel insights into nanoscopic molecular mechanisms and contributes much to the communities of protein biology. In this article, we review the recent advances in AFM-based single-protein analysis from several facets (including morphological imaging, single-molecule force recognition, mechanical unfolding pathways, and high-speed AFM molecular detection), and provide perspectives for future progression.

Language英语
WOS SubjectBiochemical Research Methods ; Nanoscience & Nanotechnology
WOS KeywordMEMBRANE-PROTEINS ; SUBNANOMETER RESOLUTION ; SIMULTANEOUS TOPOGRAPHY ; MOLECULAR-INTERACTIONS ; CONFORMATIONAL-CHANGES ; UNFOLDING PATHWAYS ; LATERAL DIFFUSION ; PLASMA-MEMBRANE ; NATIVE PROTEINS ; CELL-MEMBRANES
WOS Research AreaBiochemistry & Molecular Biology ; Science & Technology - Other Topics
Funding ProjectNational Natural Science Foundation of China[61922081] ; National Natural Science Foundation of China[61873258] ; National Natural Science Foundation of China[U1613220] ; Research Program of Frontier Sciences CAS[ZDBS-LY-JSC043] ; Youth Innovation Promotion Association CAS[2017243]
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/26220
Collection机器人学研究室
Corresponding AuthorLi M(李密)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China
2.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China
3.University of Chinese Academy of Sciences, Beijing 100049, China
4.Department of Industrial and Manufacturing Systems Engineering, University of Hong Kong, Hong Kong
Recommended Citation
GB/T 7714
Li M,Wang YC,Liu LQ. Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins[J]. IEEE TRANSACTIONS ON NANOBIOSCIENCE,2020,19(1):78-99.
APA Li M,Wang YC,&Liu LQ.(2020).Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins.IEEE TRANSACTIONS ON NANOBIOSCIENCE,19(1),78-99.
MLA Li M,et al."Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins".IEEE TRANSACTIONS ON NANOBIOSCIENCE 19.1(2020):78-99.
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