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Cell culturing in electropolymerized hydrogel multi-layer nets fabricated in an electrokinetics microfluidic chip
Li P(李盼)1,2; Liu LQ(刘连庆)1; Zhang YZ(章昱昭)1,2; Yu HB(于海波)1; Lee, Gwo-Bin3; Wang YC(王越超)1; Li WJ(李文荣)4
Department机器人学研究室
Conference Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Conference DateNovember 11-15, 2018
Conference PlaceKaohsiung, Taiwan
Author of SourceASE Group ; et al. ; Industrial Technology Research Institute (ITRI) ; KLOE ; QuantaMatrix, Inc. ; Zeon Corporation
Source Publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Publication PlaceSan Diego, CA, USA
2018
Pages252-255
Indexed ByEI
EI Accession number20200908224881
Contribution Rank1
ISBN978-1-5108-9757-1
Keyword3D cell culture 3D hydrogel Bio-fabrication Optically-induced electrokinetics
AbstractThree-dimensional (3D) micro-engineered hydrogel biomaterials incorporating cells is a promising in vitro bio-model for tissue engineering. We report a visible-light induced layer-by-layer microfabrication method for large scale tunable 3D hydrogel network and its application in 3D cell culturing and modulation. This optically induced electropolymerization method was previously reported for two-dimensional (2D) hydrogel patterning and cell patterning. Here, we improved the fabrication process by designing a series of switching "digitally-controlled photomasks" to continuously fabricate multi-layered 3D net-like hydrogel scaffolds with tunable pores and gaps. Cell spreading and migrating behaviors were further investigated after incorporating them into this biocompatible hydrogel scaffold. This method may shine some light on the in vitro reconstruction of physiologically related bio-microenvironments.
Language英语
Document Type会议论文
Identifierhttp://ir.sia.cn/handle/173321/26356
Collection机器人学研究室
Corresponding AuthorLi WJ(李文荣)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences (CAS), Shenyang 110016, China
2.University of the Chinese Academy of Sciences, Beijing 100049, China
3.Department of Power Mechanical Engineering, National Tsinghua University, Hsinchu 300, Taiwan
4.Department of Mechanical Engineering, City University of Hong Kong, Kowloon, Hong Kong
Recommended Citation
GB/T 7714
Li P,Liu LQ,Zhang YZ,et al. Cell culturing in electropolymerized hydrogel multi-layer nets fabricated in an electrokinetics microfluidic chip[C]//ASE Group, et al., Industrial Technology Research Institute (ITRI), KLOE, QuantaMatrix, Inc., Zeon Corporation. San Diego, CA, USA:Chemical and Biological Microsystems Society,2018:252-255.
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