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Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)
Zhang YZ(章昱昭)1,2,3; Zhao JH(赵俊华)4,5; Yu HB(于海波)1,2; Li P(李盼)1,2,3; Liang WF(梁文峰)6; Liu Z(刘柱)1,2; Lee, Gwo-Bin7; Liu LQ(刘连庆)1,2; Li WJ(李文荣)1,2,8; Wang ZN(王振宁)4,5
Department机器人学研究室
Source PublicationSCIENCE ADVANCES
ISSN2375-2548
2020
Volume6Issue:32Pages:1-13
Indexed BySCI ; EI
EI Accession number20203509103339
WOS IDWOS:000560452300017
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [61925307, U1908207, 61727811, 91748212, 61973224] ; National Key R&D Program of China [2018YFB1304900, 2017YFC0908300] ; Joint Project in Key Area of Liaoning Province Natural Science Foundation [2019-KF-01-10] ; Youth Innovation Promotion Association CAS [Y201943] ; Hong Kong Research Grants Council's Joint Laboratory Funding Scheme [JLFS/E-104/18]
Abstract

Detection of free gastric cancer cells in peritoneal lavages and ascites plays a vital role in gastric cancer. However, due to the low content of cancer cells in patients' peritoneal lavages, traditional detection methods lack sensitivity and cannot satisfy clinical demand. In this study, we used an optically induced electrokinetics (OEK) microfluidic method for label-free separation and characterization of patient gastric cancer cells. This method showed high effectiveness and sensitivity. We successfully separated cancer cells from a simulated peritoneal lavage mixture of gastric cancer cell lines and peritoneal lavage cells in a ratio of 1:1000. We further separated gastric cancer cells from six patients' ascites with purity up to 71%. In addition, we measured the cell membrane capacitances, which may be used as a biomarker for gastric cancer cells. Thus, our method can be used to effectively and rapidly detect peritoneal metastasis and to acquire cellular electrical information.

Language英语
WOS SubjectMultidisciplinary Sciences
WOS KeywordGASTRIC-CANCER ; PROGNOSTIC-SIGNIFICANCE ; QUANTITATIVE DETECTION ; TUMOR-CELLS ; HIGH-PURITY ; SEPARATION ; CYTOLOGY ; MANIPULATION ; CHEMOTHERAPY ; PARTICLES
WOS Research AreaScience & Technology - Other Topics
Funding ProjectNational Natural Science Foundation of China[61925307] ; National Natural Science Foundation of China[U1908207] ; National Natural Science Foundation of China[61727811] ; National Natural Science Foundation of China[91748212] ; National Natural Science Foundation of China[61973224] ; National Key R&D Program of China[2018YFB1304900] ; National Key R&D Program of China[2017YFC0908300] ; Joint Project in Key Area of Liaoning Province Natural Science Foundation[2019-KF-01-10] ; Youth Innovation Promotion Association CAS[Y201943] ; Hong Kong Research Grants Council's Joint Laboratory Funding Scheme[JLFS/E-104/18]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/27566
Collection机器人学研究室
Corresponding AuthorYu HB(于海波); Li WJ(李文荣); Wang ZN(王振宁)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110169, China
3.University of Chinese Academy of Sciences, Beijing 100049, China
4.Department of Surgical Oncology and General Surgery, the First Hospital of China Medical University, Shenyang, China
5.Key Laboratory of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Ministry of Education, Shenyang, China
6.School of Mechanical Engineering, Shenyang Jianzhu University, Shenyang 110168, China
7.Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan
8.Department of Mechanical Engineering, City University of Hong Kong, HongKong, China.
Recommended Citation
GB/T 7714
Zhang YZ,Zhao JH,Yu HB,et al. Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)[J]. SCIENCE ADVANCES,2020,6(32):1-13.
APA Zhang YZ.,Zhao JH.,Yu HB.,Li P.,Liang WF.,...&Wang ZN.(2020).Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK).SCIENCE ADVANCES,6(32),1-13.
MLA Zhang YZ,et al."Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)".SCIENCE ADVANCES 6.32(2020):1-13.
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