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Scanning Super-Resolution Imaging in Enclosed Environment by Laser Tweezer Controlled Superlens
Wen YD(文扬东)1,2,3; Yu HB(于海波)1,2; Zhao WX(赵文秀)1,2,3; Li P(李盼)1,2,3; Wang FF(王飞飞)4; Ge ZX(葛治星)1,2,3; Wang XD(王晓朵)1,2; Liu LQ(刘连庆)1,2; Li WJ(李文荣)5
Source PublicationBiophysical Journal
Indexed BySCI
WOS IDWOS:000599427000009
Contribution Rank1
Funding OrganizationNational Natural Science Foundation of China (Project No. 61727811, 61821005, 61973298 and U1613220), the Instrument Developing Project of the Chinese Academy of Sciences (Grant No. YZ201649), the CAS FEA International Partnership Program for Creative Research Teams, the Youth Innovation Promotion Association, CAS, the Hong Kong Research Grants Council (Project No. 11205415), and the Shenzhen Municipality Science and Technology Innovation Commission (Grant No. SGDX2019081623121725

Super-resolution imaging using microspheres has attracted tremendous scientific attention recently because it has managed to overcome the diffraction limit and allowed direct optical imaging of structures below 100 nm without the aid of fluorescent microscopy. To allow imaging of specific areas on the surface of samples, the migration of the microspheres to specific locations on two-dimensional planes should be controlled to be as precise as possible. The common approach involves the attachment of microspheres on the tip of a probe. However, this technology requires additional space for the probe and could not work in an enclosed environment, e.g., in a microfluidic enclosure, thereby reducing the range of potential applications for microlens-based super-resolution imaging. Herein, we explore the use of laser trapping to manipulate microspheres to achieve super-resolution imaging in an enclosed microfluidic environment. We have demonstrated that polystyrene microsphere lenses could be manipulated to move along designated routes to image features that are smaller than the optical diffraction limit. For example, a silver nanowire with a diameter of 90 nm could be identified and imaged. In addition, a mosaic image could be constructed by fusing a sequence of images of a sample in an enclosed environment. Moreover, we have shown that it is possible to image Escherichia coli bacteria attached on the surface of an enclosed microfluidic device with this method. This technology is expected to provide additional super-resolution imaging opportunities in enclosed environments, including microfluidic, lab-on-a-chip, and organ-on-a-chip devices.

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Document Type期刊论文
Corresponding AuthorYu HB(于海波); Li WJ(李文荣)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Shenyang, China
2.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang, China
3.University of the Chinese Academy of Sciences, Beijing, China
4.Department of Chemistry, Stanford University, Stanford, California
5.Department of Mechanical Engineering, City University of Hong Kong, Hong Kong, China
Recommended Citation
GB/T 7714
Wen YD,Yu HB,Zhao WX,et al. Scanning Super-Resolution Imaging in Enclosed Environment by Laser Tweezer Controlled Superlens[J]. Biophysical Journal,2020,119(12):2451-2460.
APA Wen YD.,Yu HB.,Zhao WX.,Li P.,Wang FF.,...&Li WJ.(2020).Scanning Super-Resolution Imaging in Enclosed Environment by Laser Tweezer Controlled Superlens.Biophysical Journal,119(12),2451-2460.
MLA Wen YD,et al."Scanning Super-Resolution Imaging in Enclosed Environment by Laser Tweezer Controlled Superlens".Biophysical Journal 119.12(2020):2451-2460.
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