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Collagen-Based Thiol-Norbornene Photoclick Bio-Ink with Excellent Bioactivity and Printability
Guo K(郭凯)1,2; Wang HR(王赫然)1,2,3; Li SJ(李世杰)1,2; Zhang H(张晖)1,2,3; Li S(李松)1,2; Zhu HX(朱慧轩)1,2,3; Yang ZD(杨臻达)1,2; Zhang LM(张黎明)1,2; Chang, Peng4; Zheng XF(郑雄飞)1,2
Department机器人学研究室
Source PublicationACS Applied Materials and Interfaces
ISSN1944-8244
2021
Volume13Issue:6Pages:7037−7050
Indexed BySCI ; EI
EI Accession number20210709915548
WOS IDWOS:000621051200004
Contribution Rank1
Funding OrganizationNational Key Research and Development Project under grant 2020YFB1313100, ; Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16020803) ; National Natural Science Foundation of China (51875557) ; Research Equipment Development Program of the Chinese Academy of Sciences (YZ201545, YJKYYQ20170042, and YJKYYQ20190045) ; National High Technology Research and Development Program of China (863 Program 2015AA020312) ; National Key Research and Development Program of China (2017YFC1104900) ; Foundation of State Key Laboratory of Robotics (2017-Z16)
Keywordbioprinting norbornene-functionalized collagen neutral soluble collagen photo-cross-linkable collagen thiol-ene collagen hydrogels
Abstract

As the essential foundation of bioprinting technology, cell-laden bio-ink is confronted with the inevitable contradiction between printability and bioactivity. For example, type I collagen has been widely applied for its excellent biocompatibility; however, its relatively low self-assembly speed restricts the performance in high-precision bioprinting of cell-laden structures. In this study, we synthesize norbornene-functionalized neutral soluble collagen (NorCol) by the reaction of acid-soluble collagen (Col) and carbic anhydride in the aqueous phase. NorCol retains collagen triple-helical conformation and can be quickly orthogonally cross-linked to build a cell-laden hydrogel via a cell-friendly thiol-ene photoclick reaction. Moreover, the additional carboxyl groups produced in the reaction of carbic anhydride and collagen obviously improve the solubility of NorCol in neutral buffer and miscibility of NorCol with other polymers such as alginate and gelatin. It enables hybrid bio-ink to respond to multiple stimuli, resulting in continuous cross-linked NorCol networks in hybrid hydrogels. For the first time, the collagen with a triple helix structure and gelatin can be mixed and printed, keeping the integrity of the printed construct after gelatin's dissolution. The molecular interaction among giant collagen molecules allows NorCol hydrogel formation at a low concentration, which leads to excellent cell spreading, migration, and proliferation. These properties give NorCol flexible formability and excellent biocompatibility in temperature-, ion-, and photo-based bioprinting. We speculate that NorCol is a promising bio-ink for emerging demands in tissue engineering, regenerative medicine, and personalized therapeutics.

Language英语
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/28338
Collection机器人学研究室
Corresponding AuthorZheng XF(郑雄飞)
Affiliation1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China
2.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110169, China
3.University of Chinese Academy of Sciences, Beijing 100049, China
4.Department of Plastic and Reconstructive Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, China
Recommended Citation
GB/T 7714
Guo K,Wang HR,Li SJ,et al. Collagen-Based Thiol-Norbornene Photoclick Bio-Ink with Excellent Bioactivity and Printability[J]. ACS Applied Materials and Interfaces,2021,13(6):7037−7050.
APA Guo K.,Wang HR.,Li SJ.,Zhang H.,Li S.,...&Zheng XF.(2021).Collagen-Based Thiol-Norbornene Photoclick Bio-Ink with Excellent Bioactivity and Printability.ACS Applied Materials and Interfaces,13(6),7037−7050.
MLA Guo K,et al."Collagen-Based Thiol-Norbornene Photoclick Bio-Ink with Excellent Bioactivity and Printability".ACS Applied Materials and Interfaces 13.6(2021):7037−7050.
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