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Fluidic force microscopy for monitoring and quantifying the nucleo-cytoskeletal coupling
Zare-Eelanjegh, Elaheh1; Luchtefeld, Ines1; Li M(李密)2; Sarajlic, Edin3; Zambelli, Tomaso1
Department机器人学研究室
Source PublicationEUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
ISSN0175-7571
2021
Volume50Issue:S1Pages:202-202
Indexed BySCI
WOS IDWOS:000671622300564
Contribution Rank2
Abstract

Understanding the interactions between biochemical and mechanical cues in cellular behaviors is a key for revealing numerous biological functions and development of major diseases. This necessitates creating tools enabling in situ monitoring and quantifying such small forces in intact cells. Mechanosensory complexes at the cellular level are such a demanding situation in which the dynamic interconnectivity of the cytoskeleton, nuclear envelope, and nucleoskeleton is yet to be understood. Fluidic force microscopy (FluidFM), a force-controlled micropipette configured on an atomic force microscope (AFM) enables to manipulate living cells while delivering non-permeable sensory biomolecules into their cytosol (or nucleus) for parallel screening of molecular responses. This will be achieved owing to the versatility of FluidFM tips and gentleness of cantilever-membrane contact. Thereby, I will elaborate FluidFM combined with sophisticated time-resolved optical readouts such as fluorescencelifetime imaging microscopy (FLIM) and fluorescence resonance energy transfer (FRET)-based systems to address the dynamic mechanosensory of essential components of the linker of nucleoskeleton and cytoskeleton (LINC) complex.

Language英语
WOS SubjectBiophysics
WOS Research AreaBiophysics
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Document Type期刊论文
Identifierhttp://ir.sia.cn/handle/173321/29415
Collection机器人学研究室
Corresponding AuthorZare-Eelanjegh, Elaheh
Affiliation1.Laboratory for Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Switzerland
2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, China
3.MESA+ Institute for Nanotechnology, University of Twente, Netherlands
Recommended Citation
GB/T 7714
Zare-Eelanjegh, Elaheh,Luchtefeld, Ines,Li M,et al. Fluidic force microscopy for monitoring and quantifying the nucleo-cytoskeletal coupling[J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,2021,50(S1):202-202.
APA Zare-Eelanjegh, Elaheh,Luchtefeld, Ines,Li M,Sarajlic, Edin,&Zambelli, Tomaso.(2021).Fluidic force microscopy for monitoring and quantifying the nucleo-cytoskeletal coupling.EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,50(S1),202-202.
MLA Zare-Eelanjegh, Elaheh,et al."Fluidic force microscopy for monitoring and quantifying the nucleo-cytoskeletal coupling".EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 50.S1(2021):202-202.
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