中国科学院沈阳自动化研究所机构知识库
Advanced  
SIA OpenIR  > 机器人学研究室  > 会议论文
题名: Drug-Induced Changes of Topography in Living B Lymphoma Cells: An Atomic Force Microscopy Study
作者: Li M(李密) ; Liu LQ(刘连庆) ; Xi N(席宁) ; Wang YC(王越超) ; Dong ZL(董再励) ; Yoshikazu Hirai ; Osamu Tabata ; Xiao XB(肖秀斌) ; Zhang WJ(张伟京)
作者部门: 机器人学研究室
会议名称: The First International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale
会议日期: August 29 - September 2, 2011
会议地点: Changchun, China
会议主办者: National Natural Science Foundation of China
会议录: The First International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale
出版日期: 2011
页码: 4 pp.
关键词: atomic force microscopy ; B lymphoma ; Rituximab
摘要: Due to the particular imaging mode, atomic force microscopy (AFM) provides a new technology for characterizing the topography structures of individual living cells under nearphysiological conditions. However, owing to the lack of adequate immobilization method, using AFM to visualize the topography of mammalian suspension cells is still a big challenge. In this paper, a method for imaging living mammalian suspension cells by AFM was presented. In this method, single B lymphoma suspension cells were mechanically trapped by microfabricated pillars which were coated with positively charged poly-L-lysine. Based on this immobilization, the Rituximab-induced topography changes of individual B lymphoma cells were visualized. The roughness of the cell surface’s local area before and after the stimulation of Rituximab was analysed. The force curves obtained on the cell surface before and after adding Rituximab were also analysed. The experimental results indicate the efficacy of the pillar-based immobilization and will facilitate the research of the physiological activities of living mammalian suspension cells.
产权排序: 1
内容类型: 会议论文
URI标识: http://ir.sia.cn/handle/173321/8677
Appears in Collections:机器人学研究室_会议论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
HYQW000511.pdf(1238KB)----开放获取View Download
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li M(李密)]'s Articles
[Liu LQ(刘连庆)]'s Articles
[Xi N(席宁)]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li M(李密)]‘s Articles
[Liu LQ(刘连庆)]‘s Articles
[Xi N(席宁)]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: HYQW000511.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院沈阳自动化研究所 - Feedback
Powered by CSpace