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Drug-Induced Changes of Topography in Living B Lymphoma Cells: An Atomic Force Microscopy Study
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Yoshikazu Hirai; Osamu Tabata; Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门机器人学研究室
会议名称The First International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale
会议日期August 29 - September 2, 2011
会议地点Changchun, China
会议主办者National Natural Science Foundation of China
会议录名称The First International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale
2011
页码4 pp.
产权排序1
关键词Atomic Force Microscopy b Lymphoma Rituximab
摘要Due to the particular imaging mode, atomic force microscopy (AFM) provides a new technology for characterizing the topography structures of individual living cells under nearphysiological conditions. However, owing to the lack of adequate immobilization method, using AFM to visualize the topography of mammalian suspension cells is still a big challenge. In this paper, a method for imaging living mammalian suspension cells by AFM was presented. In this method, single B lymphoma suspension cells were mechanically trapped by microfabricated pillars which were coated with positively charged poly-L-lysine. Based on this immobilization, the Rituximab-induced topography changes of individual B lymphoma cells were visualized. The roughness of the cell surface’s local area before and after the stimulation of Rituximab was analysed. The force curves obtained on the cell surface before and after adding Rituximab were also analysed. The experimental results indicate the efficacy of the pillar-based immobilization and will facilitate the research of the physiological activities of living mammalian suspension cells.
语种英语
文献类型会议论文
条目标识符http://ir.sia.cn/handle/173321/8677
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁); Zhang WJ(张伟京)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shenyang, China
2.Gruduate School of Chinese Academy of Sciences, Beijing, China
3.Department of Electrical and Computer Engineering, Michigan State University, Michigan, USA
4.Department of Micro Engineering, Kyoto University, Kyoto, Japan
5.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China
推荐引用方式
GB/T 7714
Li M,Liu LQ,Xi N,et al. Drug-Induced Changes of Topography in Living B Lymphoma Cells: An Atomic Force Microscopy Study[C]//National Natural Science Foundation of China,2011:4 pp..
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