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题名: Insulin detection based on a PDMS microfluidic system
作者: Zhan ZK(詹志坤) ; Yao P(姚萍) ; Dong ZL(董再励) ; Tung, Steve ; Hohnbaum, Jacob ; Srinivasan, Balaji ; Li WR(李文荣)
作者部门: 机器人学研究室
会议名称: 4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
会议日期: December 5-9, 2010
会议地点: Hong Kong/Macau, China
会议主办者: IEEE Nanotechnology Council; Chinese International NEMS Society; City University of Hong Kong; The Chinese University of Hong Kong; The University of Hong Kong; University of Macau
会议录: 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
会议录出版者: IEEE Computer Society
会议录出版地: Piscataway, NJ
出版日期: 2010
页码: 112-116
收录类别: EI
ISBN号: 9781612841533
关键词: Chemical detection ; Chemiluminescence ; Hydrogen peroxide ; Immunology ; Insulin ; Microchannels ; Microfluidics ; Mixers (machinery) ; Pneumatics ; Pumps
摘要: An integrated polydimethysiloxane (PDMS) microfluidic system which is composed of two pneumatic micropumps and one micromixer is developed for high-accuracy detection of insulin. The detection method is based on coupling the highly specific technique of 'double-antibody sandwich immunoassay' with the sensitive chemiluminescence of Luminol-Hydrogen Peroxide (H2O 2). The immune reactions and other related processes are carried out in the microfluidic system semi automatically. Sample transportation in the microfluidic system is accomplished by two pneumatic PDMS micropumps. Chemiluminescent measurement is conducted in a separate PDMS micromixer using a double-channel syringe pump to inject reagents. Light emitting from this mixer is detected by a highly sensitive photometer when the chemiluminescent regents flow through the mixer chamber. The results indicate that at an actuation pressure of 10psi, a mixer actuation frequency of 5Hz, and an injecting flow rate of 0.5 ml/min, the detection limit of the microfluidic system for insulin is about 10-10 M. 
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内容类型: 会议论文
URI标识: http://ir.sia.cn/handle/173321/8743
Appears in Collections:机器人学研究室_会议论文

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