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题名: Investigation of a PDMS based micromixer for heterogeneous immunoassays of insulin
作者: Zhan ZK(詹志坤) ; Qu YL(曲艳丽) ; Wang S(王帅) ; Dong ZL(董再励) ; Srinivasan, Balaji ; Tung, Stev ; Li WR(李文荣)
作者部门: 机器人学研究室
会议名称: 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
会议日期: January 20-23, 2010
会议地点: Xiamen, China
会议录: 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
会议录出版者: IEEE Computer Society
会议录出版地: Piscataway, NJ
出版日期: 2010
页码: 616-619
收录类别: EI
ISBN号: 978-1-4244-6543-9
关键词: Antigens ; Chemical detection ; Chemiluminescence ; Hydrogen peroxide ; Immunology ; Insulin ; Microchannels ; Silicones
摘要: An experimental study was carried out to evaluate the performance of a polydimethylsiloxane (PDMS) based micromixer for heterogeneous immunoassays of insulin. The detection method is based on coupling the highly specific double-antibody sandwich immunoassay with the sensitive chemiluminescence of the Luminol-Hydrogen Peroxide reaction. Both the immune and chemiluminescence reactions are conducted in the micromixer, and the resultant light emission is detected by a highly sensitive photometer. The behavior of the luminol based chemiluminescence is parabolic with respect to time, and the maximum light intensity is used here to represent the output of the reaction. The current results indicate that at an actuation pressure of 15psi, an actuation frequency of 30Hz leads to the best reaction result. At these operating conditions, the detection limit of insulin is about 10-14mol/L while the total reaction time of the insulin immunoassay in the micromixer is less than 5 minutes. ©2010 IEEE.
语种: 英语
产权排序: 1
内容类型: 会议论文
URI标识: http://ir.sia.cn/handle/173321/8746
Appears in Collections:机器人学研究室_会议论文

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Recommended Citation:
詹志坤; 曲艳丽; 王帅; 董再励; Srinivasan, Balaji; Tung, Stev; 李文荣.Investigation of a PDMS based micromixer for heterogeneous immunoassays of insulin.见:IEEE Computer Society.2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010,Piscataway, NJ ,2010,616-619
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