SIA OpenIR  > 机器人学研究室
Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy
其他题名基于AFM的药物刺激前后淋巴瘤活细胞的形貌及弹性的变化
Li M(李密); Liu LQ(刘连庆); Xi N(席宁); Wang YC(王越超); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
作者部门机器人学研究室
关键词Atomic Force Microscopy Lymphoma Elasticity Force Curve Youngʹs Modulus
发表期刊物理化学学报
ISSN1000-6818
2012
卷号28期号:6页码:1502-1508
收录类别SCI ; CSCD
WOS记录号WOS:000305035700031
CSCD记录号CSCD:4546448
产权排序1
资助机构The project was supported by the National Natural Science Foundation of China(60904095,61175103); National High Technology Research and Development Program of China(863)(2009AA03Z316); Chinese Academy of Sciences Foreign Experts Affairs International Partnership Program for Creative Research Teams
摘要Atomic force microscopy(AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions.However,owing to the lack of adequate cellular immobilization methods,AFM imaging of living,suspended mammalian cells is still a big challenge.In this paper,a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine.In this way,the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically.When the cell is stimulated by 0.2 mg·mL-1Rituximab for 2 h,the cell topography becomes more corrugated and Young smodulus decreases from 196 to 183 kPa.When the cell is stimulated by 0.5 mg·mL-1Rituximab for 2 h,thecell topography changes more significantly and some tubercles appear,and Young s modulus decreasesfrom 234 to 175 kPa.These results thus provide a unique insight into the effects of Rituximab on individualcells. 
其他摘要原子力显微镜(AFM)的发明为研究单个活细胞的形貌结构及物理特性提供了新的技术手段.然而,由于缺少合适的固定方法,利用AFM对动物悬浮活细胞的形貌进行高分辨率成像还面临着巨大的挑战.本文提出一种基于微柱阵列和静电吸附相结合的动物悬浮细胞固定方法.通过微柱阵列的机械钳制和多聚赖氨酸的静电吸附实现了对单个淋巴瘤B细胞的固定,并在此基础上利用AFM动态观测了不同浓度Rituximab刺激下淋巴瘤B细胞的表面形貌及弹性的变化.经过0.2 mg?mL-1的Rituximab刺激2 h后,细胞表面的褶皱增加,细胞的杨氏模量从196 kPa减小到183 kPa.经过0.5 mg?mL-1的Rituximab刺激2 h后,细胞形貌发生显著变化并出现突起结构,细胞的杨氏模量从234 kPa减小到175 kPa.实验结果表明淋巴瘤细胞形貌和弹性变化的幅度随着Rituximab刺激浓度的增加而增加,加深了对Rituximab作用效果的认识.
语种英语
WOS标题词Science & Technology ; Physical Sciences
WOS类目Chemistry, Physical
关键词[WOS]FIBROBLASTS ; INHIBITION
WOS研究方向Chemistry
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
被引频次:7[CSCD]   [CSCD记录]
文献类型期刊论文
条目标识符http://ir.sia.cn/handle/173321/9989
专题机器人学研究室
通讯作者Liu LQ(刘连庆); Xi N(席宁); Zhang WJ(张伟京)
作者单位1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, P. R. China
2.Graduate University of Chinese Academy of Sciences, Beijing 100049, P. R. China
3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing 48824, USA
4.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, P. R. China
推荐引用方式
GB/T 7714
Li M,Liu LQ,Xi N,et al. Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy[J]. 物理化学学报,2012,28(6):1502-1508.
APA Li M.,Liu LQ.,Xi N.,Wang YC.,Dong ZL.,...&Zhang WJ.(2012).Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy.物理化学学报,28(6),1502-1508.
MLA Li M,et al."Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy".物理化学学报 28.6(2012):1502-1508.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Drug-Induced Changes(4018KB)期刊论文作者接受稿开放获取ODC PDDL浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li M(李密)]的文章
[Liu LQ(刘连庆)]的文章
[Xi N(席宁)]的文章
百度学术
百度学术中相似的文章
[Li M(李密)]的文章
[Liu LQ(刘连庆)]的文章
[Xi N(席宁)]的文章
必应学术
必应学术中相似的文章
[Li M(李密)]的文章
[Liu LQ(刘连庆)]的文章
[Xi N(席宁)]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。