中国科学院沈阳自动化研究所机构知识库
Advanced  
SIA OpenIR  > 机器人学研究室  > 期刊论文
题名: Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy
其他题名: 基于AFM的药物刺激前后淋巴瘤活细胞的形貌及弹性的变化
作者: Li M(李密) ; Liu LQ(刘连庆) ; Xi N(席宁) ; Wang YC(王越超) ; Dong ZL(董再励) ; Xiao XB(肖秀斌) ; Zhang WJ(张伟京)
作者部门: 机器人学研究室
关键词: Atomic force microscopy ; Lymphoma ; Elasticity ; Force curve ; Youngʹs modulus
刊名: 物理化学学报
ISSN号: 1000-6818
出版日期: 2012
卷号: 28, 期号:6, 页码:1502-1508
收录类别: SCI ; CSCD
产权排序: 1
摘要: 原子力显微镜(AFM)的发明为研究单个活细胞的形貌结构及物理特性提供了新的技术手段.然而,由于缺少合适的固定方法,利用AFM对动物悬浮活细胞的形貌进行高分辨率成像还面临着巨大的挑战.本文提出一种基于微柱阵列和静电吸附相结合的动物悬浮细胞固定方法.通过微柱阵列的机械钳制和多聚赖氨酸的静电吸附实现了对单个淋巴瘤B细胞的固定,并在此基础上利用AFM动态观测了不同浓度Rituximab刺激下淋巴瘤B细胞的表面形貌及弹性的变化.经过0.2 mg?mL-1的Rituximab刺激2 h后,细胞表面的褶皱增加,细胞的杨氏模量从196 kPa减小到183 kPa.经过0.5 mg?mL-1的Rituximab刺激2 h后,细胞形貌发生显著变化并出现突起结构,细胞的杨氏模量从234 kPa减小到175 kPa.实验结果表明淋巴瘤细胞形貌和弹性变化的幅度随着Rituximab刺激浓度的增加而增加,加深了对Rituximab作用效果的认识.
英文摘要: Atomic force microscopy(AFM) provides a means for characterizing the surface topography and biophysical properties of individual living cells under near-physiological conditions.However,owing to the lack of adequate cellular immobilization methods,AFM imaging of living,suspended mammalian cells is still a big challenge.In this paper,a method is presented for immobilizing individual living B lymphoma cells that combines mechanical trapping with pillar arrays and electrostatic adsorption with poly-L-lysine.In this way,the topography and elasticity changes of individual B lymphoma cells that were stimulated with different concentrations of Rituximab were observed and measured dynamically.When the cell is stimulated by 0.2 mg·mL-1Rituximab for 2 h,the cell topography becomes more corrugated and Young smodulus decreases from 196 to 183 kPa.When the cell is stimulated by 0.5 mg·mL-1Rituximab for 2 h,thecell topography changes more significantly and some tubercles appear,and Young s modulus decreasesfrom 234 to 175 kPa.These results thus provide a unique insight into the effects of Rituximab on individualcells. 
WOS记录号: WOS:000305035700031
WOS标题词: Science & Technology ; Physical Sciences
类目[WOS]: Chemistry, Physical
关键词[WOS]: FIBROBLASTS ; INHIBITION
研究领域[WOS]: Chemistry
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sia.cn/handle/173321/9989
Appears in Collections:机器人学研究室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy.pdf(4018KB)----开放获取View Download
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Li M(李密)]'s Articles
[Liu LQ(刘连庆)]'s Articles
[Xi N(席宁)]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Li M(李密)]‘s Articles
[Liu LQ(刘连庆)]‘s Articles
[Xi N(席宁)]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Drug-Induced Changes of Topography and Elasticity in Living B Lymphoma Cells Based on Atomic Force Microscopy.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院沈阳自动化研究所 - Feedback
Powered by CSpace